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Technology Name
Briefcase
Scientist
1780
A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and...

A method based on Fast Neutron Resonance Transmission (FNRT) radiography that enables determining weight percentages of oil and water in thick, intact cores taken from subterranean or underwater geological formations. As part of geological exploitation to find oil and water, cores are extracted and tested to determine oil/water content.
This new method allows determining such content rapidly, in non- destructive, specific and quantities analysis of the cores.

Applications


  • Determining the identity and proportions of substances of oil and water content and their distribution in inspected cores

Advantages


  • A non-destructive method which enables to determine the fluid content along the entire length of an intact core or aggregate of cores within their protective sleeves.
  • More comprehensive information and considerable saving of analysis time compared to conventional sampling methods.
    Suitable for all types of rocks including tight-shale rocks.
  • This method enables to measure the weight fraction of oil and water in the core regardless of the core shape, thickness or distribution.
  • The fluid weight fractions in the samples are determined independently, thus the ratio of oil-to-rock weight-ratio is independent of the water content.
  • Due to high penetration of fast neutrons, the method is suitable for screening intact thick rock cores (10-15 cm), for which alternative probes, such as X-rays or slow neutrons suffer limited penetration.

Technology's Essence


In order to map the oil and water content and their distribution, an aggregate of intact cores within their protective sleeves is positioned on a moving conveyor belt and scanned by a broad- energy, fast- neutron beam. The neutrons are detected by a spectroscopic fast neutron imaging detector. The map of neutron-transmission spectra in each pixel provides information of oil/water content and distribution in such cores. 

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  • Prof. Amos Breskin
1556
Synthetic carbon fixation pathways can allow plants to produce more biomass using the same amount of energy from sunlight. Novel carbon fixation cycles discovered at The Weizmann Institute hold potential to greatly increase the capacity of organisms to convert atmospheric carbon into sugars. Modern...

Synthetic carbon fixation pathways can allow plants to produce more biomass using the same amount of energy from sunlight. Novel carbon fixation cycles discovered at The Weizmann Institute hold potential to greatly increase the capacity of organisms to convert atmospheric carbon into sugars.

Modern agriculture faces limited arable land and climate changes. Carbon fixation under these conditions will become a significant growth limiting factor. The proposed solution provides the ability to enhance crop yields using the same expanse of land.

The novel technology presents alternative synthetic carbon fixation pathways that were discovered by harnessing a systems biology approach. These pathways are predicted to harbor a significant kinetic advantage over their natural counter parts, making them promising candidates for synthetic biology implementation.

Applications


  • Synthetic organisms utilizing this revolutionary technology can offer higher carbon fixation rates as compared to natural alternatives allowing:
  • Superior rate of biomass generation, providing cost effective feedstock for the production of biofuels.
  • Enhanced food production via increased crop yields.

Advantages


  • Minimal thermodynamic bottlenecks and superior kinetics over natural counterparts.

Technology's Essence


The productivity of carbon fixation cycles is limited by the slow rate and lack of substrate specificity of the carboxylating enzyme, RuBisCo. In his discovery Dr. Milo addresses the inefficiency of the carbon fixation process through an alternative cycle that is predicted to be two to three times faster than the Calvin–Benson cycle, employing the most effective carboxylating enzyme, phosphoenolpyruvate carboxylase, using the core of the naturally evolved C4 cycle.

A computational strategy was applied, comparing kinetics, energetic and topology of all the possible pathways that can be assembled from all ~4,000 metabolic enzymes known in nature.

The results suggest a promising new family of synthetic carbon fixation pathways.

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  • Prof. Ron Milo
1571
A novel social behavior monitoring system automatically tracks the precise location of each animal at excellent temporal resolution. This innovative technology provides simultaneous identification of complex social and individual behaviors via an integration of RFID and video surveillance. There is a...

A novel social behavior monitoring system automatically tracks the precise location of each animal at excellent temporal resolution. This innovative technology provides simultaneous identification of complex social and individual behaviors via an integration of RFID and video surveillance.

There is a rapidly growing interest in detecting the molecular substrates of social behavior. This interest is driven by the vast implications of such understanding in both research and the pharmaceutical industry, since some prevalent pathological conditions are mainly characterized by a behavioral deficit or abnormality.

It is extremely challenging to quantify social behavior in a reliable manner. Existing methods struggle to find a balance between objectively quantifying behavior on one hand while enabling a natural, stress-free behavioral estimation on the other hand. Currently, researchers work in a strictly controlled and constrained environment that is estranged and stressful to the animals. The outcome is a highly contaminated measurement of natural behavior. This difficultly becomes increasingly complex when more than one animal is involved as often applied in social behavioral studies.

Applications


  • Rigorous characterization of social organizational deficiencies and evaluation of their severity in animal and human models (for example in autism).
  • An optimized system for estimating the efficacy of clinical treatments.

Advantages


  • Long-term tracking of unlimited number of simultaneously studied animals.
  • Machine based, hence objective and automated quantification of behavior.
  • Excellent spatiotemporal resolution in semi natural environment
  • Flexible- the number, size and distribution of the RFID antennas can be adjusted with different enclosure dimensions.
  • Can be applied from Individual behavioral profile or pairs interactions up to collective social organization of groups.
  • Systematic analysis and classification of basic locomotion up to more complex social

Technology's Essence


Researchers at the Weizmann institute developed a method for tightly controlled monitoring of social behavior in a semi-natural environment. They used integrated and synchronized chip reporting and continuous video postage to precisely locate each individual animal. Using this automated monitoring which provides an exceptional temporal resolution they achieved correct identification of numerous basic individual behaviors as well as complex social behaviors. Such complex behavioral profiles set the basis for subsequent analysis which reveals the formation of a social hierarchy.

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  • Dr. Tali Kimchi
358
Escherichia coli UTL2 Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane. Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane...

Escherichia coli UTL2

Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane.

Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane permeability mutant of Escherichia coli. Proc Natl Acad Sci U S A 93(12):5969-74.

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  • Prof. Eitan Bibi
1378
Researchers at the Weizmann Institute developed a novel method to design error-free DNA libraries from error-prone oligonucleotides. The system surpasses existing methods for de novo synthesis of DNA libraries in speed, precision, amenability to automation and ease of combining synthetic with natural...

Researchers at the Weizmann Institute developed a novel method to design error-free DNA libraries from error-prone oligonucleotides. The system surpasses existing methods for de novo synthesis of DNA libraries in speed, precision, amenability to automation and ease of combining synthetic with natural DNA fragments. 

All DNA construction protocols struggle with the cumbersome task of cloning and sequencing synthetic DNA fragments, seeking an error-free one. The problem is worsened for longer synthetic DNA which is more prone to errors. Time spent on error correction, clone selection and sequencing is a major bottleneck that prevents de novo DNA synthesis from becoming a routine procedure in labs. 

This innovative solution significantly decreases the need for labor-intensive time-consuming error correction methods, cloning and sequencing. Furthermore, efficient editing and reassembly of different genes is made possible due to a smart recursive reconstruction process.

 

Applications


  • Design and construction of synthetic biological molecules and organisms.
  • Construction of designer DNA libraries.

 


Advantages


  • Applicable in any lab with standard lab equipment. Faster and more precise than existing methods.
  • Amenable to automation, full synthesis in vitro with a modified smPCR protocol.
  • Very simple to combine synthetic and natural DNA fragments.
  • Does not require additional or external methods or reagents for error correction

 


Technology's Essence


Divide and Conquer (D&C), the quintessential recursive problem-solving technique, is applied in silico to divide the target DNA sequence into overlapping oligonucleotides short enough to be synthesized directly, albeit with errors; error-prone oligonucleotides are recursively combined in vitro, forming error-prone DNA molecules; error-free fragments of these molecules are then identified, extracted and used as new, typically longer and more accurate, inputs to another iteration of the recursive construction procedure; the entire process repeats until an error-free target molecule is formed.

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  • Prof. Ehud Y. Shapiro
1166
A series of monoclonal antibodies for monitoring hormone and drug additives in animals grown for the food industry. These include mAbs for peptide hormones, steroid hormones, drugs, leukotrienes, isoflavones, and veterinary drugs.

A series of monoclonal antibodies for monitoring hormone and drug additives in animals grown for the food industry. These include mAbs for peptide hormones, steroid hormones, drugs, leukotrienes, isoflavones, and veterinary drugs.

Applications


Monitoring hormone and drug additives in food providing animals for veterinary use and for the food industry.

Technology's Essence


Researchers at the Weizmann Institute of Science have developed a series of mAb against peptide and steroid hormones, isoflavones, and human and veterinary drugs. These antibodies are particularly valuable for monitoring hormone and drug additives in food providing animals. The mAb are available for diagnostics, research, and therapeutics.

The following mAb are available for licensing:

(Clones marked with * are available for diagnostic and therapeutic use only).

Peptide Hormones:
LH: 4F10
bFSH: 1G12*, 1H9, 1H7
FSH: 6H6
bHCG: 1D5
bHCG+: 1C7 3F11
HGH: 1C12*, 1C4*, 5E9, 4E12, 5C3, 1C5, 6G3, 5E6, 2C12

Steroid Hormones:
progesterone-11a-HS 1E11*
progesterone-7a-CET 2H4
Estrone-3-glucuronide 8A3
Testosterone-3-CMO 5A4
Testosterone-3-CMO 5F2*
Estradiol-6-CMO 8D9*

Anti-idiotypic antibodies to anti-steroids:
betatypic anti-anti-testosterone 5A4 8G9
betatypic anti-progesterone 2H4 15F11
betatypic anti-anti-estrone-3-glucuronide 8A3 7C1
alphatypic anti-progesterone 2H4 2E11
betatypic anti-anti-estrone-3-glucuronide 8A3 11C1

Drugs
Digoxin 10F10
RU-486* 8B6*
Buserelin 8B4
Medroxy-progesterone-acetate* 1F5*

Leukotrienes
LTC4* 6E7

Biotin
Biotin-BSA F1

Isoflavones
Daidzein 4E4
Daidzein/daidzin/genistin 2F11
Estrone-3-glucuronide 8A3
Genistein/biochanin A 10D8
Genistein/genistin/daidzin 6E8
Betatypic anti-anti-genistein 10D8

Veterinary drugs
Sulfamethazine (SMZ) 21C7
Betatypic anti-SMZ 12E12
4-chloro-androstenedione 14H2
Virginamycin 486
Spiramycin 110
Betatypic anti-anti-spiramycin 133

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  • Dr. Fortune Kohen
261
261 - Monoclonal antibody to GluR3B Description: Mouse monoclonal antibody (IgG2a), raised against a synthetic peptide of the Glutamate receptor (GluR3B), corresponding to amino acids 383-395 (NEYERFVPFSDQQISNDSSSSENR) of Leishmania donovani GluR3B. May be used for diagnosis as well as drug...

261 - Monoclonal antibody to GluR3B

Description: Mouse monoclonal antibody (IgG2a), raised against a synthetic peptide of the Glutamate receptor (GluR3B), corresponding to amino acids 383-395 (NEYERFVPFSDQQISNDSSSSENR) of Leishmania donovani GluR3B.

May be used for diagnosis as well as drug development for "autoimmune epilepsy”, a condition characterized by high levels of neuropathogenic human anti-GluR3B in serum and CSF.

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  • Dr. Mia Levite
  • Prof. Vivian I. Teichberg
118
  Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry. Leukotrienes:   Drugs: §  118 - Monoclonal antibody to Buserelin      Description: Rat...

 

Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs

May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.

Leukotrienes:

 

Drugs:

§  118 - Monoclonal antibody to Buserelin

     Description: Rat monoclonal antibodies raised against Buserelin.

     Available clone: 8B4, IgG1.

 
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  • Dr. Fortune Kohen
176
176-177 - Monoclonal antibodies to phospho-ERK/Map Kinase Description: Monoclonal antibodies raised against peptides containing the 11 amino acids, HTGFLTEYVAT, corresponding to the ERK activation loop either Tyr -phosphorylated (ERK-PY193), or Thr-phosphorylated (ERK-PT115). ERK belongs to the...

176-177 - Monoclonal antibodies to phospho-ERK/Map Kinase

Description: Monoclonal antibodies raised against peptides containing the 11 amino acids, HTGFLTEYVAT, corresponding to the ERK activation loop either Tyr -phosphorylated (ERK-PY193), or Thr-phosphorylated (ERK-PT115).

ERK belongs to the family of mitogen-activated protein kinases (MAPKs) and is an important component in many intracellular signaling events. ERK is phosphorylated and activated by the upstream kinases, MEK1 and MEK2 on regulatory Threonin (Thr) and tyrosine (Tyr) residues, which are localized in the activation loop of ERK.

Reference: Yao Z, Dolginov Y, Hanoch T, Yung Y, Ridner G, Lando Z, Zharhary D, Seger R. 2000. Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468(1):37-42.

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  • Prof. Rony Seger
295
295 – Milstein catalyst Description: Carbonylhydrido[6-(di-t-butyl-phosphinomethylene)-2-(N,N-diethylaminomethyl)-1,6-dihydropyridine]ruthenium(II). Ruthenium-based catalyst that converts amines and alcohols into amides. Reference: Chidambaram Gunanathan, Yehoshoa Ben-David, David Milstein. 2007....

295 – Milstein catalyst

Description: Carbonylhydrido[6-(di-t-butyl-phosphinomethylene)-2-(N,N-diethylaminomethyl)-1,6-dihydropyridine]ruthenium(II).

Ruthenium-based catalyst that converts amines and alcohols into amides.

Reference: Chidambaram Gunanathan, Yehoshoa Ben-David, David Milstein. 2007. Direct Synthesis of Amides from Alcohols and Amines with Liberation of H2. Science

317(5839):790-2.

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  • Prof. David Milstein
143
Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry. Leukotrienes: Drugs: §  143 - Monoclonal antibody to Medroxy-progesterone-acetate     ...

Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs

May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.

Leukotrienes:

Drugs:

§  143 - Monoclonal antibody to Medroxy-progesterone-acetate

     Description: Rat monoclonal antibodies raised against Medroxy-progesterone- acetate(MPA)-3-carboxymethyl oxime- BSA. Available clone: 1F5, IgG1.

     Medroxy-progesterone-acetate (MPA) is a highly potent progestational steroid that has been used worldwide as a contraceptive.

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  • Dr. Fortune Kohen
238
238 - CP Peptide Description: Core peptide (CP; GLRILLLKV-D-stereoisomer), a synthetic peptide coding for the transmembrane domain of the ?-subunit of the T-cell receptor (TCR), a region that has been identified to be crucial for the assembly and function of the TCR. Was shown to significantly inhibit...

238 - CP Peptide

Description: Core peptide (CP; GLRILLLKV-D-stereoisomer), a synthetic peptide coding for the transmembrane domain of the ?-subunit of the T-cell receptor (TCR), a region that has been identified to be crucial for the assembly and function of the TCR.

Was shown to significantly inhibit T-cell-mediated inflammation upon subcutaneously administration in animal models. Has the potential of being used as an alternative therapy for immunosuppression.

Reference: Gerber D, Quintana FJ, Bloch I, Cohen IR, Shai Y. 2005. D-enantiomer peptide of the TCRalpha transmembrane domain inhibits T-cell activation in vitro and in vivo. FASEB J. 19(9):1190-2.

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  • Prof. Yechiel Shai
265
Monoclonal antibodies specific to cholesterol/ceramide mixture Description: Monoclonal antibodies specific for cholesterol/ceramide-rich domains (clones 405F, 14F, 499F) and cholesterol micro-domains (clones 36A1, 5881) in cell membranes. Originally raised against an artificial monolayer of lipid...

Monoclonal antibodies specific to cholesterol/ceramide mixture

Description: Monoclonal antibodies specific for cholesterol/ceramide-rich domains (clones 405F, 14F, 499F) and cholesterol micro-domains (clones 36A1, 5881) in cell membranes.

Originally raised against an artificial monolayer of lipid mixtures in, and were shown to specifically label the above domains in different cell membranes.

Reference:  Scheffer L, Futerman AH, Addadi L. 2007. Antibody labeling of cholesterol/ceramide ordered domains in cell membranes. Chembiochem 8(18):2286-94.

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  • Prof. Lia Addadi
120
Monoclonal antibodies raised against bFSH. Available clone: 1G12. Follicle-stimulating hormone (FSH) is synthesized and secreted by gonadotrophs of the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation and reproductive processes of the body.Reference: Somjen D1,...

Monoclonal antibodies raised against bFSH. Available clone: 1G12.
Follicle-stimulating hormone (FSH) is synthesized and secreted by gonadotrophs of the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation and reproductive processes of the body.
Reference: Somjen D1, Tordjman K, Kohen F, Baz M, Razon N, Ouaknine G, Stern N. 1997. Combined beta FSH and beta LH response to TRH in patients with clinically non-functioning pituitary adenomas. Clin Endocrinol (Oxf). 46(5):555-62.

Monoclonal antibodies for peptide and steroid hormones

Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.

 

Peptide Hormones:

§  120 – Monoclonal antibody to FSH

Description: Monoclonal antibodies raised against bFSH. Available clone: 1G12.

Follicle-stimulating hormone (FSH) is synthesized and secreted by gonadotrophs of the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation and reproductive processes of the body.

      Reference: Somjen D1, Tordjman K, Kohen F, Baz M, Razon N, Ouaknine G, Stern N. 1997. Combined beta FSH and beta LH response to TRH in patients with clinically non-functioning pituitary adenomas. Clin Endocrinol (Oxf). 46(5):555-62.


 

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  • Dr. Fortune Kohen
179
179 - Allyl-mercapto-captopril (CPSSA - Captosal) Description: Conjugation between allicin (the biologically active molecule in garlic) and captopril, a known antihypertensive drug. Was shown to offer therapeutic benefits for the treatment of the metabolic syndrome, as demonstrated by various animal...

179 - Allyl-mercapto-captopril (CPSSA - Captosal)

Description: Conjugation between allicin (the biologically active molecule in garlic) and captopril, a known antihypertensive drug. Was shown to offer therapeutic benefits for the treatment of the metabolic syndrome, as demonstrated by various animal models. These include improvement of glucose tolerance, preventing weight gain, lowering blood pressure, reducing cardiac hypertrophy and potent antioxidant activities.

Reference: Miron T, Rabinkov A, Peleg E, Rosenthal T, Mirelman D, Wilcheck M. 2004. Allylmercaptocaptopril: a new antihypertensive drug. Am. J. Hypertens. 1:71-73.

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  • Prof. David Mirelman

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