You are here

Category
Technology Name
Briefcase
Scientist
1673
CF is the most common autosomal recessive disorder in western countries, affecting approximately 30,000 people in the US alone. A major risk in CF arises from chronic bacterial lung infections, affecting 80% of CF patients by the age of 25. Bacterial lung infections are also of major clinical...

CF is the most common autosomal recessive disorder in western countries, affecting approximately 30,000 people in the US alone. A major risk in CF arises from chronic bacterial lung infections, affecting 80% of CF patients by the age of 25. Bacterial lung infections are also of major clinical importance in patients with chronic obstructive pulmonary disease (COPD), trauma, burn wounds, sepsis, or in patients requiring ventilation. The infections are currently treated with antibiotics, which rapidly become inefficient as resistant bacteria strains arise. The present technology suggests a novel therapeutic approach for the prevention and treatment of bacterial lung infection in susceptible populations, especially CF patients

Applications


  • Alternative treatment for bacterial lung infections.
  • A prophylaxis for patients susceptible to bacterial lung infections

Advantages


  • A novel therapeutic approach to prevent or cure bacterial lung infection.
  • The new therapy is based on reinforcement of the physiological innate immunity rather than on antibiotics.
  • The new therapy can be easily administered, via inhalation.
  • FTY720, a SPH analog, is already in clinical use for treating multiple sclerosis.

Technology's Essence


Sphingosine (SPH), a natural bactericidal agent which acts as a part of the human innate immune system in the skin, was found to be an effective treatment and prophylaxis for bacterial lung infections in cystic fibrosis (CF) mice. The new technology is based on the discovery that both CF human patients and CF mice display reduced rates of SPH in the airways. Moreover, normalizing SPH levels by inhalation prevents or cures the infections in CF mice, thus rendering SPH and its analogs a potent therapeutic agent for CF patients, an alternative to antibiotics.

+
  • Prof. Anthony H. Futerman
1733
The spatial distribution of proteins inside the cell is under tight regulation. This regulation is necessary to ensure proper functioning of the cell, and is of particular importance when extracellular stimulation is applied. Upon stimulation, many signaling proteins rapidly and dynamically change...

The spatial distribution of proteins inside the cell is under tight regulation. This regulation is necessary to ensure proper functioning of the cell, and is of particular importance when extracellular stimulation is applied. Upon stimulation, many signaling proteins rapidly and dynamically change their location. Today, there is a widely recognized need to identify novel sequences which regulates nuclear translocation.
Recently, Prof. Zeger and his team discovered a new level of regulation to stimulated transcription. They showed that ?-like importunes are central mediators of nuclear translocation of signaling proteins. Furthermore they identified the site of interaction and designed accordingly a peptide which was found to prevent nuclear translocation.
This technology presents peptides with the potential of treating inflammatory and immune disease by regulating (prevent or promote) the translocation of proteins into the nucleus.

Applications


  • Inflammation
  • Immune diseases

Advantages


  • Effective
  • Safe

Technology's Essence


The researchers found that ?-like importins play a key role in JNK and p38 translocation. They also found that the translocation of these MAPKs is mediated by the formation of either Imp3/Imp7/MAPK or Imp3/Imp9MAPK heterodimers. Most importantly, the researchers identified the site in p38 that mediate the interaction with Imp7 and Imp9 and showed that the important sequence lies within residues 20-30 of p38?. Subsequently they synthesized a 14 amino acid myristoylated peptide based on the sequence of residues 21-34 of p38?. When it was applied to HeLa cells prior to stimulation, it prevented the nuclear translocation and Imp7/9 interaction of the MAPKs. Since the peptides of this technology are able to specifically inhibit the nuclear activities of p38 (such as inflammatory activities) without modulating their cytoplasmic activities, these peptides may serve as a therapeutic agent for inflammatory and apoptosis related diseases without having side effect.

 

+
  • Prof. Rony Seger
1684
Gaseous energy sources such as hydrogen and natural gas (predominantly methane) encompass an intrinsic transport problem because of their volatility and flammability. Adsorption of the gas on a solid material (such as MOF) facilitates safe, light and economical transport of the gas. This is especially...

Gaseous energy sources such as hydrogen and natural gas (predominantly methane) encompass an intrinsic transport problem because of their volatility and flammability. Adsorption of the gas on a solid material (such as MOF) facilitates safe, light and economical transport of the gas. This is especially significant in the huge natural gas (NG) market where solutions are required for storage and transport of the gas whether from NG reservoirs in high pressure giant tanks or as a compact low pressure NG tank for small vehicles and other NG powered devices.
The invention involves a new method for the formation of uniform metal organic frameworks (MOFs) at quantitative yields and in a controlled manner.
These MOFs can be tailored to adsorb specific gases for low pressure - high volume storage and transport applications.

Applications


  • Low pressure – high volume gas storage and transportation
  • Safe storage of toxic or otherwise dangerous gases
  • Low energy solid phase gas separation and purification
  • Production of MOF-based catalysts

Advantages


  • Uniform crystallite morphology
  • A quantitative process
  • Ability to design and control product structure
  • Control of pore size
  • Single step process
  • No additives

Technology's Essence


The invention comprises a new solvothermal synthetic procedure in which specific metal ions are selected to react with specific organic ligands to form uniform sub-microstructured MOFs with a narrow size distribution and without the need for a modulator to define the crystal morphology.
Controlling the selected reagents as well as the specific reaction conditions influences the resulting crystallites formed and enables a fine selection of the desired structure.
MOFs prepared this way have exceptional uniformity profiles of size and shape and can be tailored to selectively adsorb specific gases for low pressure - high volume storage and transport applications.

+
  • Prof. Milko E. Van der Boom
1802
A new signal processing tool for the detection of pulses travelling through media with complex or unknown dispersion properties was developed by the group of Prof. Gal-Yam, originally for detecting radio bursts in astronomical observations. Pulses are applied in various fields such as oil & gas...

A new signal processing tool for the detection of pulses travelling through media with complex or unknown dispersion properties was developed by the group of Prof. Gal-Yam, originally for detecting radio bursts in astronomical observations.
Pulses are applied in various fields such as oil & gas exploration, detection (e.g. sonar, lidar and radar) and communication. When pulses pass through dispersive media, the arrival times at the detector of different frequency components may differ, and as a result the pulse may become degraded (e.g. transformed to a longer pulse with reduced intensity), even to the level of becoming indistinguishable in terms of signal to noise. This problem becomes even more challenging when detecting short pulses that travel through complex or unknown media.
The new method presented here provides a proven and efficient solution that can be applied for different scenarios where short pulses dispersed by complex media are used. 

Applications


  • Detection and surveying technologies- sonar, lidar, radar etc

Advantages


  • Efficient, requires limited computational resources
  • Generic, can be applied to various setups
  • Easily implementable into existing systems

Technology's Essence


The method includes obtaining an input array of cells, each indicating an intensity of a frequency component of the signal at a representative time. A fast dispersion measure transform (FDMT) is applied to concurrently sum the cells of the input array that lie along different dispersion curves, each curve defined by a known non-linear functional form and being uniquely characterized by a time coordinate and by a value of the dispersion measure. Application of FDMT includes initially generating a plurality of sub-arrays, each representing a frequency sub-band and iteratively combining pairs of adjacent sub-arrays in accordance with an addition rule until all of the initially generated plurality of sub-arrays are combined into an output array of the sums, in which a cell of the output array that is indicative of a transmitted pulse is identified.

+
  • Prof. Avishay Gal-Yam
1751
Many cancer cells hijack and remodel existing metabolic pathways for their benefit. Specific targeting of these metabolic dependencies offers cancer patients increased efficiency and minimized side effects. Yet, the complexity of these pathways hinders the identification of targets. The present...

Many cancer cells hijack and remodel existing metabolic pathways for their benefit. Specific targeting of these metabolic dependencies offers cancer patients increased efficiency and minimized side effects. Yet, the complexity of these pathways hinders the identification of targets.
The present discovery elucidates the pathway by which argininosuccinate synthase (ASS1) down-regulation confer cancer progression. It shows that decreased activity of ASS1 in cancers supports proliferation by linking excess aspartate to pyrimidines synthesis. Importantly, these studies highlight Citrin (a mitochondrial aspartate transporter) inhibition as a potential method to decrease aspartate levels and selectively target this metabolic pathway in ASS1 depleted cancers.

Applications


  • Targeted Treatment for ASS1 depleted cancers.

Advantages


  • Targeted therapy, against a well defined pathway, increases the prospects for success.
  • Selective – targeting cancer metabolic dependency minimizes the chances for healthy cells damage that lead to side effects.

Technology's Essence


Cancer cells hijack and remodel existing metabolic pathways for their benefit in what is termed the Warburg effect. Researchers from Dr. Ayelet Erez's lab, at the Weizmann institute of Science, have delineated the metabolic benefit(s) conferred by loss of ASS1 to cancers. In agreement with previous experience, they found that ASS1 deficiency has an additional arginine- independent effect that is directly related to its substrate, aspartate.
By focusing on the relevant physiological and pathological model systems, it was found that ASS1 deficiency-mediated increase in aspartate levels lead to excessive proliferation through pyrimidine synthesis. The link between the two is provided by CAD (carbamoyl-phosphate synthase 2, aspartate transcarbamylase, dihydroorotase complex) and the mTOR signaling pathway.
Importantly, the present inventors have found that blocking Citrin, the mitochondrial aspartate transporter, rescues cell proliferation by reducing aspartate levels. Citrin may thus serve as a strong candidate for targeted therapy of ASS1 depleted cancers.   
Supporting this model, retrospective survival analysis of several cancers reveal that cancers with both decreased ASS1 expression and high Citrin levels have a trend for significantly worse prognosis.

+
  • Dr. Ayelet Erez
1712
  • Prof. Yechiel Shai
1753
The Chiral Induced Spin Selectivity (CISS) effect, discovered in recent years by Prof. Ron Naaman from the Weizmann Institute of Science, implies that electrons transferred through chiral molecules possess a specific spin orientation. Hence, the molecular chirality and electron spin are correlated.A...

The Chiral Induced Spin Selectivity (CISS) effect, discovered in recent years by Prof. Ron Naaman from the Weizmann Institute of Science, implies that electrons transferred through chiral molecules possess a specific spin orientation. Hence, the molecular chirality and electron spin are correlated.
A team of researchers lead by Prof. Naaman have been investigating the CISS effect in different systems. They found that the high efficiency of many natural multiple electron reactions can also be attributed to spin alignment of the electrons involved.
The present innovation looks at hydrogen production through water electrolysis, showing that when using anodes coated by chiral molecules the efficiency of the electrolysis process increases by 30% compared to using uncoated, regular electrodes.

Applications


  • Control of electron spin
  • Significant reduction of over-potential in spin sensitive electrochemical reactions
  • Efficient electrochemical processes
  • Minimum side reactions

  • Advantages


     

    Technology's Essence


    Spin selective electrodes made from standard electrode material are coated with chiral molecules. These coated electrodes were used for electrolysis of water and showed superior efficacy compared to standard un-coated electrodes, by reduction of the over-potential required for the process. This is explained by the spin selective electron conduction through the chiral layer:

     

     

     

    Hydrogen production as a function of time for (A) the chiral molecules and (B) for the achiral molecules. The potentials in the brackets refer to the over-potential compared to DNA coated electrode. The measurements were conducted at the Eapp for each of the molecules.

     

    +
    • Prof. Ron Naaman
    1670
    A method for selective extraction of precious and rare metals has been developed at the Weizmann Institute. This method allows the efficient and environmentally benign recovery of precious materials that are currently discarded of in large quantities from spent catalysts (automotive and industrial)...

    A method for selective extraction of precious and rare metals has been developed at the Weizmann Institute. This method allows the efficient and environmentally benign recovery of precious materials that are currently discarded of in large quantities from spent catalysts (automotive and industrial) from industrial processes (particularly in the electronic industry).

    Prof. Igor Lubomirsky’s novel process is based on volatilization for selective extraction of precious and rare metals using benign metal salts, rather than dangerous chlorine gas as a chlorinating agent. The new process requires relatively low temperatures and is free from hazardous waste, among its additional advantages over conventional methods.

    We believe that this efficient technology is key to increased reclaimed precious metals output, potentially resulting in the reduction of the demand for primary rare metals.

    Applications


    ·           Recycling precious metals from spent items, e.g. platinum group metals from catalytic convertors


    Advantages


    ·         No toxic input – chlorides are used rather than chlorine gas.

    ·         No hazardous waste is generated in the process.

    ·         Mild conditions. High-temperature furnaces and equipment are not required.

    ·         Relatively simple setup in comparison to conventional ones.

    ·         Small scale plants are economically viable.


    Technology's Essence


    Prof. Igor Lubomirsky and his group developed a novel method for the recovery of PGM from spent catalysts that can be applicable for other spent systems as well.

    The method comprises of crushing the spent catalyst to obtain a catalyst particulate material with g a predetermined grain size and reacting it with chlorine containing salts rather than pure chlorine gas in a furnace at relatively low temperatures (900oC, far below the temperature required in the conventional volatilization method). This is followed by cooling the volatile PMG chloride product converting it into solid phase metal.

     

    +
    • Prof. Igor Lubomirsky
    • Prof. Igor Lubomirsky
    1717
    Converting two low-energy photons into a single higher-energy photon is of significant importance in many fields. In medical imaging, photon up-conversion is used for imaging scattered specimens, while in photovoltaic devices it could be used to harvest photons with energies lower than the bandgap of...

    Converting two low-energy photons into a single higher-energy photon is of significant importance in many fields. In medical imaging, photon up-conversion is used for imaging scattered specimens, while in photovoltaic devices it could be used to harvest photons with energies lower than the bandgap of the absorber.
    Currently available systems, based on rare-earth-doped dielectrics, and organic materials are limited in both tunability and absorption cross-section. In fact, no known up-conversion systems operate on photons in the 1000-1500 nm range.
    Stable inorganic nanocrystalline up-conversion systems designed at the Weizmann Institute of Science provide broad tunability of both the absorption edge and the luminescence color. These materials have the potential to be utilized in applications such as high-energy photon sources, photovoltaics and IR detection.

    Applications


    • Easy to manufacture

    • Robust systems

    • Operation at room temperature


    Advantages


    • Photon sources

    • Photovoltaics

    • IR detectors


    Technology's Essence


    The new up-conversion systems are based on a novel design comprising a compound semiconductor nanocrystal, which incorporates two quantum dots with different bandgaps separated by a tunneling barrier. The expected up-conversion mechanism occurs by the sequential absorption of two photons. The first photon excites an electron–hole pair by interband absorption in the lower-energy core, resulting in a confined hole and a relatively delocalized electron. The second absorbed photon leads to further excitation of the hole, allowing it to cross the barrier layer. This, in turn, is followed by radiative recombination with the delocalized electron.

    +
    • Prof. Dan Oron
    1772
    MTCH2 as a novel target for the treatment of obesity.Obesity is an escalating public health problem with an increasing prevalence worldwide, and a primary contingency of many life-threatening diseases, as well as early mortality. In the U.S. alone, more than one-third of adults are obese. Obesity-...

    MTCH2 as a novel target for the treatment of obesity.
    Obesity is an escalating public health problem with an increasing prevalence worldwide, and a primary contingency of many life-threatening diseases, as well as early mortality. In the U.S. alone, more than one-third of adults are obese. Obesity-related conditions include heart disease, stroke, type 2 diabetes and certain types of cancer, some of the leading causes of preventable death. Physicians and patients alike consider the weight-loss efficacy of the current therapeutics to be unsatisfactory. Therefore, there is an unmet need for innovative options that are at once safe and efficacious, and allow the patient to maintain weight loss.
    The present invention describes the identification of Mitochondrial Carrier Homolog 2 (MTCH2) as a novel player in muscle metabolism and the therapeutic potential of inhibiting MTCH2 for the treatment of diet-induced obesity and diabetes.

    Advantages


    • A fresh approach for targeting weight-related disorders
    • Direct effect on metabolism instead of indirect mechanisms of current therapeutics which target appetite modulation.
    • Protection from diet-induced obesity can be used as a prevention treatment for people with a tendency for weight gain.  

    Technology's Essence


    MTCH2 functions as a receptor-like protein for the pro-apoptotic BID protein in the mitochondria.
    MTCH2 was identified as one of six new gene loci associated with Body Mass Index (BMI) and obesity in humans suggesting that MTCH2 may also play a role in metabolism.
    MTCH2 was recently shown by the Gross’s lab to also function as a repressor of   mitochondria oxidative phosphorylation (OXPHOS) in the hematopoietic system.
    Deletion of MTCH2 in skeletal muscle increases mitochondrial OXPHOS and mass, and increases capacity for endurance exercise. In addition, loss of MTCH2 increases mitochondria and glycolytic flux in muscles as measured by monitoring pyruvate and lactate levels.
    MTCH2 knockout mice are protected from diet-induced obesity, hyperinsulinemia, and are more prone to weight loss upon caloric restriction.
    Therefore, the association of MTCH2 with mitochondrial function offers a potential novel target for muscle metabolism modulation in the fight against metabolic disorders such as obesity and diabetes.

     

    +
    1672
    Newly developed p53-reactivating peptides were shown to cause regression of very aggressive tumors in several cancer models. p53 is the most important  tumor suppressor gene. Mutant p53 forms can instigate a cascade of events that may lead to loss of control of cell growth and proliferation, and...

    Newly developed p53-reactivating peptides were shown to cause regression of very aggressive tumors in several cancer models. p53 is the most important  tumor suppressor gene. Mutant p53 forms can instigate a cascade of events that may lead to loss of control of cell growth and proliferation, and eventually to cancer. p53 is mutated in well over 50% of all tumors, making it the most frequently known mutated gene in human cancer. Most conventional chemotherapeutic and radio-therapeutic treatments activate p53 pathway and therefore have little effect on tumors harboring mutant-p53 forms. The p53 reactivating peptides are capable of changing the conformation of many forms of mutated p53 from mutant to wild-type form and restoring its tumor suppression functions. Hence, they have the potential to benefit up to 50% of cancer patients – patients who currently have very poor response to the conventional cancer treatments.

    Applications


    ·         A potential first line therapy for mutant p53-bearing cancers

    ·         A potential combination treatment with Chemotherapy and other means of cancer therapy


    Advantages


    ·         Suitable for treatment of about 50% of cancer patients, in which conventional treatments show poor results.

    ·         Based on restoring the natural tumor-suppression mechanisms


    Technology's Essence


    The proposed technology is based on the development of peptides that are capable of changing the conformation of p53 from mutant to wild-type form and restoring its tumor suppression functions. The peptides were generated using Phage Display technique and screened so that only peptides capable of shifting the equilibrium towards the wild type conformation of p53 were selected. Preliminary pre-clinical experiments show that these p53-reactivating peptides cause regression of very aggressive tumors in several cancer models. Hence, the p53 reactivating peptides have the potential to benefit up to 50% of cancer patients – patients who currently have very poor response to the conventional cancer treatments.

    +
    • Prof. Varda Rotter
    • Prof. Moshe Oren
    1730
    Production of carbon nanotube based transistors through a process comprised of identification, selection, and placement of pristine carbon nanotubes in conjunction with standard electrical circuitry.Semiconductor devices are vital to everyday life, however conventional semiconducting materials are...

    Production of carbon nanotube based transistors through a process comprised of identification, selection, and placement of pristine carbon nanotubes in conjunction with standard electrical circuitry.
    Semiconductor devices are vital to everyday life, however conventional semiconducting materials are quickly approaching their limitations. As devices transition from the microscale to the nanoscale, new techniques for their assembly and testing of their properties must be created. Controllable nanofabrication methods are of increasing importance across a wide field of electronics in everything from energy efficient LEDs in flat-screen monitors to transistors for ultra-powerful computers. Our process presents a novel method for producing high quality nanoscale carbon nanotube based transistors. These methods will be of the utmost importance in the forthcoming nano-revolution.

    Applications


    • Produce flawless carbon nanotubes
    • Identify, select, and position nanotubes with precision
    • Room temperature operation
    • High sensitivity
    • High resolution

    Advantages


    • Single electron transistor (SET) nanoscale imaging
    • Novel nano-electromechanical devices

    Technology's Essence


    The principle behind this technology is two-fold: 1) Synthesis and selection method of flawless carbon nanotubes, and 2) their combination with nanoscale electric circuitry to form fully controlled composite nanoscale electronic device.
    Selection of the carbon nanotube(s) is assisted by a scanning probe microscope (SPM). A composite electronic device is assembled from two separated chips; a nanotube chip where nanotubes are grown over wide trenches, and a standard circuit chip with electrode contacts surrounding the gates to be measured. The nano-assembly is achieved by inserting an SPM cantilever into a trench on the nanotube chip and placing the circuit chip over a suitable nanotube. Once in place, the nanotube is cut locally by passing a strong current between the electrode contacts, and the composite chip is formed.
    This composite electronic device can be used to map electronic potentials with high resolution of 100 nm, high sensitivity of 1microV/Hz1/2, at frequencies of 100 MHz and more and all this at room temperature.

    +
    • Prof. Shahal Ilani
    1801
    A new image processing tool for transient detection was developed by the group of Prof. Gal-Yam, originally for time-domain observational astronomy.Image sequences are used in various fields, including medical imaging and satellite/airborne imaging. The comparison between images taken at different...

    A new image processing tool for transient detection was developed by the group of Prof. Gal-Yam, originally for time-domain observational astronomy.
    Image sequences are used in various fields, including medical imaging and satellite/airborne imaging. The comparison between images taken at different conditions (e.g. equipment or configuration, angles, weather and wavelength) can be a highly non-trivial problem, as subtraction artifacts can outnumber real changes between images.
    The existing remedy for this problem includes highly complex solutions using machine learning algorithms to narrow the sea of candidates. In some cases, human interpretation of images cannot be avoided, resulting is very long processing times.
    The new method presented here provides a proven solution for the subtraction of images taken at varying conditions. The tool can be applied for any type of imaging, allowing fast processing and accurate results.

    Applications


    • Satellite/airborne imaging

    • Medical imaging
    • Defect detection

    Advantages


    • Fast and automatic

    • Generic, can be applied to various imaging scenarios
    • Easily implementable into existing systems

    Technology's Essence


    The new method is used for processing at least two N-dimensional data-measurements (DMs) of a physical-property for detecting one or more new-objects and/or a transition of one or more known-objects, in complex constant-background DMs. Generally, the

    the method includes: (1) generating a filtered-new-DM by match-filtering a new-DM, respective to impulse response of a reference-DM (2) generating a filtered-reference-DM by match-filtering the reference-DM, respective to impulse response of the new-DM (3) generating an N-dimensional object-indicator (OI) by subtracting the filtered-reference-DM from the filtered-new-DM, or vice versa and (4) generating an N-dimensional data score from the N-dimensional OI, where each of the scores is a probe for existence of an object at a specific N-dimensional location.
    +
    • Prof. Avishay Gal-Yam
    1679
    A novel therapy for Triple Negative Breast Cancer (TNBC) using mAbs combinationBreast cancer is the most common cancer in women worldwide. Triple-negative breast cancer (TNBC) representing about 15% of all breast cancer cases, is the deadliest form of all breast cancer subtypes, and tends to affect...

    A novel therapy for Triple Negative Breast Cancer (TNBC) using mAbs combination
    Breast cancer is the most common cancer in women worldwide. Triple-negative breast cancer (TNBC) representing about 15% of all breast cancer cases, is the deadliest form of all breast cancer subtypes, and tends to affect women at a younger age. Unfortunately TNBC cannot be treated with the common receptor targeted therapies since it does not express these targets, the estrogen, progesterone and Her2/neu receptors. Therefor systemic treatment options are currently limited to cytotoxic chemotherapy. The lack of effective targeted therapies, resistance to chemotherapy, and early metastatic spread have contributed to the poor prognoses and outcomes associated with TNBC.
    The current technology offers a novel therapeutic strategy for TNBC. The application of two novel, noncompetitive antibodies against EGFR, achieves a robust degradation EGFR resulting in tumor inhibition.

    Applications


    • Novel and unique antibody targeted therapy for TNBC.
    • The novel anti EGFR antibodies can cooperate synergistically with the currently marketed EGFR antibodies.

    Advantages


    • A promising therapeutic scenario to treat TNBC.
    • Enhanced EGFR degradation and improved anti-tumor activity, in contrast to clinically approved anti-EGFR mAbs, which display no cooperative effects.
    • Lysosomal EGFR degradation pathway induced by epitope-distinct antibody mixture may potentially lead to improved therapeutic outcome, and reduced resistance.

    Technology's Essence


    Prof. Yosef Yarden and his team demonstrated that a combination of novel antibodies that target distinct regions on the human EGF receptor resulted in its robust and synergistic down-regulation, leading to pronounced tumor growth inhibition. Furthermore, the combined mAbs induced lysosomal degradation of EGFR, while avoiding the recycling route. Such irreversible mode of EGFR degradation may potentially increase response rate or delay the onset of patient resistance.
    Conversely, combining cetuximab and panitumumab, the mAbs routinely used to treat colorectal cancer patients, did not improve receptor degradation because they are both attracted to the same epitope on EGFR.

    +
    • Prof. Yosef Yarden
    1750
    Organophosphates are toxic compounds found in chemical warfare agents, such as nerve gases, and insect pesticides.Use of volatile nerve gas agents by terrorist organizations is a key concern of governments around the world. V-type nerve agents (e.g. VX, RVX, and CVX) are particularly toxic nerve gases...

    Organophosphates are toxic compounds found in chemical warfare agents, such as nerve gases, and insect pesticides.
    Use of volatile nerve gas agents by terrorist organizations is a key concern of governments around the world. V-type nerve agents (e.g. VX, RVX, and CVX) are particularly toxic nerve gases, with an exceptionally high potency. Although not as lethal as nerve agents, organophosphate insecticides can be harmful in large or prolonged doses. The standard therapy has limited efficacy, carry risks of serious adverse effects and have relatively short shelf life in field conditions.
    Bioscavengers represent a preferred to rapidly detoxify organophosphates in the blood, before they had the chance to reach its physiological targets and cause damage, but usually require the use of very high doses.
    The present invention provides genetically modified phosphotriesterase (PTE) variants, which serve as catalytic bioscavengers for V-type nerve agents, with exceptional detoxification activity at low doses, and improved stability.

    Applications


    • Prophylactic or post exposure treatment for nerve gases attack, in particular V-type agents
    • Treatment for pesticides poisoning

    Advantages


    • High catalytic activity – allow high efficacy at low doses
    • Reduced effective doses allows to reduce adverse effects
    • High stability increasing shelf life
    • Compatible with both prophylaxis and post exposure
    • Compatible for both surface decontamination and administration to patients

    Technology's Essence


    Researchers at Prof. Tawfik lab use directed evolution to drive protein mutagenesis towards desired traits. Appling this approach, using the actual threat agents, the present inventors generated recombinant phosphotriesterase (PTE) variants with improved catalytic efficiencies towards V-type nerve agent hydrolysis. Serving as catalytic bioscavengers, these recombinant PTE variants hydrolyze organophosphates without being consumed and thus can be applied at low doses (catalytic efficiency (kcat/KM) greater than 3.106 M-1min-1).
    Importantly, PTE is efficient both as a prophylactic agent that may be given several hours prior to exposure as a preventive measure, and as post exposure antidote, even days after in a single or multiple-doses.
    It is compatible with both decontamination of surfaces and detoxification administrated to a patient by standard routes such as orally or injectables.
    Finally, some PTE variants show superior stability properties, retaining at least 50% of their catalytic activity at 50?C, indicating extended shelf life. This may be especially critical in field conditions, where the risk for nerve agent exposure is high.

    +
    • Prof. Dan S. Tawfik

    Pages