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Technology Name
Briefcase
Scientist
358
Escherichia coli UTL2 Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane. Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane...

Escherichia coli UTL2

Description: A "leaky" strain of E. coli, which is significantly more susceptible to cytotoxic agents. UTL2 holds a mutation in the galU gene causing an impaired outer membrane.

Reference:  B?j? O, Bibi E. 1996. Functional expression of mouse Mdr1 in an outer membrane permeability mutant of Escherichia coli. Proc Natl Acad Sci U S A 93(12):5969-74.

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  • Prof. Eitan Bibi
1448
A method to produce amides in one step without any unwanted by-products, by coupling of alcohols with amines with the liberation of hydrogen gas, catalyzed by unique ruthenium complexes. Amides are widely used in the industry (e.g. nylon, Kevlar) and have widespread importance in biochemical and...

A method to produce amides in one step without any unwanted by-products, by coupling of alcohols with amines with the liberation of hydrogen gas, catalyzed by unique ruthenium complexes.

Amides are widely used in the industry (e.g. nylon, Kevlar) and have widespread importance in biochemical and chemical systems (e.g. proteins). Synthesis of amides is mostly based on activated acid derivatives or rearrangement reactions induced by an acid or base, which often produce toxic chemical waste and involve tedious procedures. Therefore, an efficient synthesis that avoids wasteful use of coupling reagents or corrosive acidic and basic media is highly desirable. The current technology allows for the clean production of amides from amines and alcohols.

Applications


  • Production of amides for various applications (plastic and rubber industry, paper industry, pharmaceutical intermediates, etc.)

  • Use of the liberated hydrogen (e.g. for the production of ammonia)


Advantages


  • Clean and selective procedure

  • Environment friendly reaction (no base or acid promoters are required, no carboxylic acid derivatives, such as acid chlorides, are needed)

  • Amides and molecular hydrogen are produced in high yields and high turnover numbers directly from alcohols in one step

  • The liberated hydrogen can be used for different applications

  • Formation of a variety of amides


Technology's Essence


Amide formation is a fundamental reaction in chemical synthesis. Amides are commonly formed from the reaction of a carboxylic acid derivative with an amine. Instead of using carboxylic acid derivative, in the present invention the amide motif is generated by direct acylation of amines with alcohols. This is possible through the use of a unique catalyst. This method enables the simple and elegant production of amide polymers and industrially important amides.

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  • Prof. David Milstein
1270
Monoclonal antibodies to IgE Description: Rat monoclonal anti-IgE antibodies that was generated by fusion of plasmacytoma (84.1C) or myeloma (EM953) cells with splenocytes of rat immunized with purified murine IgE mAb. The antibodies react with various IgE mAb of different specificities and not with...

Monoclonal antibodies to IgE

Description: Rat monoclonal anti-IgE antibodies that was generated by fusion of plasmacytoma (84.1C) or myeloma (EM953) cells with splenocytes of rat immunized with purified murine IgE mAb. The antibodies react with various IgE mAb of different specificities and not with immunoglobulins of other classes, and recognize an epitope on the murine Fc epsilon region.

Were shown to block IgE-Fc?R interactions and inhibit passive cutaneous anaphylaxis. 

Clone 84.1c recognizes a site on IgE, which is identical or very close to the Fc?R binding site. May be used for detection and manipulation of the IgE response in mice.

Reference:  Schwarzbaum S, Nissim A, Alkalay I, Ghozi MC, Schindler DG, Bergman Y, Eshhar Z. 1989. Mapping of murine IgE epitopes involved in IgE-Fc epsilon receptor interactions. Eur J Immunol 19(6):1015-23.

 

M182, M185, M186

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  • Prof. Zelig Eshhar
1238
Coherently combining two or more low-power laser beams preserves low-power beam quality while significantly boosting the overall beam power as a function of the number of individual beams. The result is a high-power, high-quality robust laser beam.

Coherently combining two or more low-power laser beams preserves low-power beam quality while significantly boosting the overall beam power as a function of the number of individual beams. The result is a high-power, high-quality robust laser beam.

Applications


Coherent combining of laser beams leads to compact, stable and robust, laser configurations with very high output powers and good beam quality. Such laser configuration could be potentially exploited in many important medical and industrial applications. These include laser material processing, such as laser welding and laser cutting, laser surgery and laser pollution monitoring.

Technology's Essence


At the Weizmann Institute of Science, a team of researchers developed a novel approach for intra-cavity phase locking and coherent addition of two or more laser beam distributions. This includes the phase locking and coherent addition of Gaussian distributions, single high-order mode distributions, and even spatially incoherent multimode distributions. In their approach the phase locking and coherent addition is achieved using new interferometric and polarization couplers/combiners with a variety of laser configurations. The laser configurations are compact and robust, enabling stable operation under harsh environmental conditions. Some successful preliminary experiments were performed with pulsed Nd:YAG lasers. In these experiments phase locking and coherent addition of several laser distributions were demonstrated, with more than 95% combining efficiency and excellent beam quality. The overall configuration was found to be very stable and insensitive to geometrical displacements of the combiner coupler.

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  • Prof. Asher A. Friesem
  • Prof. Nir Davidson
1506
A simple electrochemical method and apparatus for the continues production of CO (carbon monoxide) from CO2 as chemical storage for electrical energy and a basic material for further organic products. Constant progress is made in solar and wind alternative energy production. Unfortunately, these...

A simple electrochemical method and apparatus for the continues production of CO (carbon monoxide) from CO2 as chemical storage for electrical energy and a basic material for further organic products.

Constant progress is made in solar and wind alternative energy production. Unfortunately, these systems are weather and time-dependent. Additionally, most of the geographic areas best suited for harvesting these resources are remote from population centers. Therefore the need for a reliable method to store and transport renewable energy is clear.

CO can be easily converted into methanol, which is one of the major chemical raw materials and can by itself be used as fuel for diesel engines and the energy source for direct methanol fuel cells (DMFC).

At present no reliable method of CO2 to CO reduction is available. Either using low temperatures which leads to low thermodynamic efficiency (<60%), Requires precious metals for electrodes and results in toxic byproducts, or using high temperatures which Requires pure CO2 input and Produces a mixture of CO2 and CO.

The current technology describes an efficient, flexible, continues method for production of CO at high temperatures (900oC) without any byproducts or toxic materials.

Applications


  • Production of CO from CO2
  • Easy conversion into methanol

Advantages


·         No precious (Pt, Ag, Au, Pd) metals required

·         No hazardous chemicals involved, no pollution

·         Continuous operation is possible

·         One can use flue gas as a source

·         Capture of CO2 from air is possible

·         The system is very compact>20 kW/m3

·         Operation conditions are very flexible

·         The process fits existing infrastructure

·         CO can be easily converted into liquid fuel (CH3OH)


Technology's Essence


The outlined technology overcomes the basic problems of CO production by using molten Li2CO3 as the electrolyte, a Ti container (will not undergo corrosion), Ti cathode (does not catalyze decomposition of CO), and a graphite anode (no chemical reaction with Li2CO3). At 900°C and current density of 0.05-2 A/cm2, this unique system enables a thermodynamic efficiency close to 100%, continues production of CO – efficiently separating CO2 to CO and O2.

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  • Prof. Igor Lubomirsky
1378
Researchers at the Weizmann Institute developed a novel method to design error-free DNA libraries from error-prone oligonucleotides. The system surpasses existing methods for de novo synthesis of DNA libraries in speed, precision, amenability to automation and ease of combining synthetic with natural...

Researchers at the Weizmann Institute developed a novel method to design error-free DNA libraries from error-prone oligonucleotides. The system surpasses existing methods for de novo synthesis of DNA libraries in speed, precision, amenability to automation and ease of combining synthetic with natural DNA fragments. 

All DNA construction protocols struggle with the cumbersome task of cloning and sequencing synthetic DNA fragments, seeking an error-free one. The problem is worsened for longer synthetic DNA which is more prone to errors. Time spent on error correction, clone selection and sequencing is a major bottleneck that prevents de novo DNA synthesis from becoming a routine procedure in labs. 

This innovative solution significantly decreases the need for labor-intensive time-consuming error correction methods, cloning and sequencing. Furthermore, efficient editing and reassembly of different genes is made possible due to a smart recursive reconstruction process.

 

Applications


  • Design and construction of synthetic biological molecules and organisms.
  • Construction of designer DNA libraries.

 


Advantages


  • Applicable in any lab with standard lab equipment. Faster and more precise than existing methods.
  • Amenable to automation, full synthesis in vitro with a modified smPCR protocol.
  • Very simple to combine synthetic and natural DNA fragments.
  • Does not require additional or external methods or reagents for error correction

 


Technology's Essence


Divide and Conquer (D&C), the quintessential recursive problem-solving technique, is applied in silico to divide the target DNA sequence into overlapping oligonucleotides short enough to be synthesized directly, albeit with errors; error-prone oligonucleotides are recursively combined in vitro, forming error-prone DNA molecules; error-free fragments of these molecules are then identified, extracted and used as new, typically longer and more accurate, inputs to another iteration of the recursive construction procedure; the entire process repeats until an error-free target molecule is formed.

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  • Prof. Ehud Y. Shapiro
1033
A novel diagnostic test to identify individuals with increased risk of lung cancer. Lung cancer is the number one killer among cancers, with 160,000 deaths/year in the USA and 1.6 million/year worldwide. Early detection of lung cancer increases 5-year survival rate from 4% to 54%. Moreover, the...

A novel diagnostic test to identify individuals with increased risk of lung cancer.

Lung cancer is the number one killer among cancers, with 160,000 deaths/year in the USA and 1.6 million/year worldwide. Early detection of lung cancer increases 5-year survival rate from 4% to 54%. Moreover, the National Lung Cancer Trial (NLST) showed that early detection of lung cancer by low-dose CT reduces mortality by at least 20%. Despite recommendations for low-dose CT screening for heavy smokers fulfilling the NLST criteria, compliance is low. In addition, 80 million smokers and ex-smokers in the US who do not fulfil NLST risk criteria have no recommended solution.

The MyRepair test fulfils this unmet medical need by providing a quantitative prediction of lung cancer risk using a simple blood test. The test is based on a personalized measurement of the patient’s DNA repair capacity, a mechanism which is highly connected to the onset of cancer. Therefore, the MyRepair technology can potentially increase early detection of lung cancer and thus save lives.

 

Applications


A novel diagnostic test to identify individuals with increased risk of lung cancer


Advantages


·         Simplicity – MyRepair is based on a simple, cost-effective blood test.

·         Accessibility – Compared to low-dose CT which requires specific equipment, the MyRepair test can be easily integrated in general diagnostic labs and therefore may be more accessible to a larger portion of the population.

·         Additional applications – Since the test is based on measuring personalized DNA repair mechanism, it can be adopted in the future for the diagnosis of additional cancer types and DNA repair related diseases.


Technology's Essence


Based on the strong and well documented connection between impaired capacity for DNA repair and onset of cancer, the Livneh lab invented the MyRepair Test, a method for predicting lung cancer risk, based on measuring activity of 3 DNA repair enzymes.

Combining enzyme activities with experimental risk estimates generated MyRepair Score, which measures personalized DNA repair capacity of tested subjects.

An epidemiological/clinical study performed in Israel, further validated in an independent UK study, demonstrated that lung cancer patients have lower MyRepair Score than healthy people. In addition, subjects who test MyRepair-positive have an 85-fold higher risk to develop lung cancer compared to the general population.

Low MyRepair Score is a risk factor independent of smoking, and of comparable magnitude, indicating that it can be a prognostic tool for smokers, ex-smokers, and non-smokers.

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  • Prof. Zvi Livneh
1451
A monoclonal antibody against GluR3B, a peptide found in epilepsy patients, and especially in patients suffering from intractable, resistant forms of the disease, could be used in diagnosis kits as well as in drug development for this form of "autoimmune epilepsy".

A monoclonal antibody against GluR3B, a peptide found in epilepsy patients, and especially in patients suffering from intractable, resistant forms of the disease, could be used in diagnosis kits as well as in drug development for this form of "autoimmune epilepsy".

Applications


1. Producing a new kit for epilepsy patients, able to detect GluR3b Ab's and thus GluR3-mediated neuropathology
The anti GluR3B monoclonal Ab could be used for developing a new diagnostic kit to detect neuropathogenic human anti-GluR3B in serum and CSF of patients with epilepsy. The patient's GluR3B Ab's would compete and displace the GluR3B mAb's of its ligand: the GluR3B peptide. The presence of GluR3B Ab's in a patient, would indicate that autoimmunity against GluR3 may underlie the patient's neuropathology and a) would suggest the initiation of an immune-based therapy b) prevent useless and dangerous brain surgery c) prevent non-effective medication.

2. Drug design for GluR3-mediated neuropathology
The unique GluR3B monoclonal antibody could be used to screen a potential drug for 'Autoimmune Epilepsy'. The GluR3B monoclonal antibody could be used to screen for a molecule (i.e. Anti-idiotypic antibodies) that would block the GluR3 autoantibodies and their detrimental neuropathological effects.

3. Research tool for a kaleidoscope of purposes, including:

  • Detection of the GluR3 glutamate receptor subtype on various target cells.
  • Studies of the properties of the Glutamate/AMPA receptor subtype 3.
  • Studies of the Glutamate-liked agonist activity of the GluR3B monoclonal antibody, and of the GluR3 receptor ion channel gating properties.
  • Production of an animal model of 'Autoimmune Epilepsy'.
  • Studies of neuronal death caused by binding of the GluR3 autoantibody to glutamate/AMPA receptors.
  • Studies of behavioral impairments caused by binding of the GluR3 autoantibody to glutamate/AMPA receptors.

  • Technology's Essence


    Scientists from the Weizmann Institute of Science have discovered a unique anti-GluR3B monoclonal antibody Glu149/29/61.

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    • Prof. Vivian I. Teichberg
    • Prof. Vivian I. Teichberg
    237
    236-237 - Diastereomer Lytic Peptides for Treatment of Solid Tumors and Metastasis Description: 15-mer (Leu-Lys-Dleu- Leu-Lys-Dlys-Leu-Dleu-Dlys-Lys-Leu-Leu-Dlys-Leu-Leu) and 15-mer Histidin (H-Leu-Lys-D-Leu- Leu-His-D-Lys-Leu-D-Leu-D-Lys-His-Leu-Leu-D-Lys-Leu-Leu-NH2) are membrane-active peptides...

    236-237 - Diastereomer Lytic Peptides for Treatment of Solid Tumors and Metastasis

    Description: 15-mer (Leu-Lys-Dleu- Leu-Lys-Dlys-Leu-Dleu-Dlys-Lys-Leu-Leu-Dlys-Leu-Leu) and 15-mer Histidin (H-Leu-Lys-D-Leu- Leu-His-D-Lys-Leu-D-Leu-D-Lys-His-Leu-Leu-D-Lys-Leu-Leu-NH2) are membrane-active peptides composed of both D- and L amino acids (diastereomers). These peptides have demonstrated potent anti-cancer and anti metastatic activities in several animal models including models for prostate and lung cancer. They were shown to successfully inhibit tumor growth when injected intratumorally or intraveneously. The 15-mer Histidine form shows reduced systemic toxicity.

    References: Papo N, Braunstein A, Eshhar Z, Shai Y. 2004. Suppression of human prostate tumor growth in mice by a cytolytic D-, L-amino Acid Peptide: membrane lysis, increased necrosis, and inhibition of prostate-specific antigen secretion. Cancer Res. 64(16):5779-86.

    Makovitzki A1, Fink A, Shai Y. 2009. Suppression of human solid tumor growth in mice by intratumor and systemic inoculation of histidine-rich and pH-dependent host defense-like lytic peptides. Cancer Res. 69(8):3458-63.

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    • Prof. Yechiel Shai
    288
    hVin monoclonal antibody Description: Monoclonal antibodies raised against human Vinculin purified from human uterus. Specifically stains Vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells. Good reactivity is obtained with human, bovine, chicken, dog, rat mouse, turkey and...

    hVin monoclonal antibody

    Description: Monoclonal antibodies raised against human Vinculin purified from human uterus. Specifically stains Vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells. Good reactivity is obtained with human, bovine, chicken, dog, rat mouse, turkey and xenopus Vinculin.

    Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of

    several interacting proteins involved in anchoring F-actin to the membrane.

    Reference: Geiger B, Volk T, Volberg T, Bendori R. 1987. Molecular interactions in adherens-type contacts. J Cell Sci Suppl.8:251-72.

    Applications


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    • Prof. Benjamin Geiger
    142
    Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.   Biotin: §  116, 142 – Monoclonal antibody to Biotin            Description: Monoclonal...

    Monoclonal antibodies for Isoflavones, leukotrienes, biotin and human and veterinary drugs

    May be used for monitoring drug additives in food providing animals for veterinary use and for the food industry.

     

    Biotin:

    §  116, 142 – Monoclonal antibody to Biotin

               Description: Monoclonal antibodies raised against biotinylated BSA.

               Available clones: F1, F4.

    Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). Functions as coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.

    Reference: Bag?i H1, Kohen F, Kus?uoglu U, Bayer EA, Wilchek M. 1993. Monoclonal anti-biotin antibodies simulate avidin in the recognition of biotin. FEBS Lett. 322(1):47-50.

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    • Dr. Fortune Kohen
    263
    Description: Monoclonal antibodies specific for cholesterol/ceramide-rich domains (clones 405F, 14F, 499F) and cholesterol micro-domains (clones 36A1, 5881) in cell membranes. Originally raised against an artificial monolayer of lipid mixtures in, and were shown to specifically label the above domains...

    Description: Monoclonal antibodies specific for cholesterol/ceramide-rich domains (clones 405F, 14F, 499F) and cholesterol micro-domains (clones 36A1, 5881) in cell membranes.
    Originally raised against an artificial monolayer of lipid mixtures in, and were shown to specifically label the above domains in different cell membranes.
    Reference:  Scheffer L, Futerman AH, Addadi L. 2007. Antibody labeling of cholesterol/ceramide ordered domains in cell membranes. Chembiochem 8(18):2286-94.

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    • Prof. Lia Addadi
    119
    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products. Rat monoclonal antibodies raised against testosterone 3-(0- carboxymethyl)oxime-BSA. Available...

    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.
    Rat monoclonal antibodies raised against testosterone 3-(0- carboxymethyl)oxime-BSA. Available clones: 5F2, 5A4.
    Testosterone is the principal male sex hormone and an anabolic steroid. In mammals, testosterone is secreted primarily in the testicles of males and the ovaries of females, although small amounts are also secreted by the adrenal glands.
    Reference: Kohen F, Lichter S, Eshhar Z, Lindner HR. 1982. Preparation of monoclonal antibodies able to discriminate between testosterone and 5 alpha-dihydrotestosterone. Steroids. 39(4):453-9.

    Monoclonal antibodies for peptide and steroid hormones

    Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. A particular use may be quality control of industrial manufacturing of food products.

    Steroid Hormones:

    §  119, 169 – Monoclonal antibodies to Testosterone          

          Description: Rat monoclonal antibodies raised against testosterone 3-(0-   carboxymethyl)oxime-BSA. Available clones: 5F2, 5A4.

    Testosterone is the principal male sex hormone and an anabolic steroid. In mammals, testosterone is secreted primarily in the testicles of males and the ovaries of females, although small amounts are also secreted by the adrenal glands.

    Reference: Kohen F, Lichter S, Eshhar Z, Lindner HR. 1982. Preparation of monoclonal antibodies able to discriminate between testosterone and 5 alpha-dihydrotestosterone. Steroids. 39(4):453-9.


     

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    • Dr. Fortune Kohen
    177
    176-177 - Monoclonal antibodies to phospho-ERK/Map Kinase Description: Monoclonal antibodies raised against peptides containing the 11 amino acids, HTGFLTEYVAT, corresponding to the ERK activation loop either Tyr -phosphorylated (ERK-PY193), or Thr-phosphorylated (ERK-PT115). ERK belongs to the...

    176-177 - Monoclonal antibodies to phospho-ERK/Map Kinase

    Description: Monoclonal antibodies raised against peptides containing the 11 amino acids, HTGFLTEYVAT, corresponding to the ERK activation loop either Tyr -phosphorylated (ERK-PY193), or Thr-phosphorylated (ERK-PT115).

    ERK belongs to the family of mitogen-activated protein kinases (MAPKs) and is an important component in many intracellular signaling events. ERK is phosphorylated and activated by the upstream kinases, MEK1 and MEK2 on regulatory Threonin (Thr) and tyrosine (Tyr) residues, which are localized in the activation loop of ERK.

    Reference: Yao Z, Dolginov Y, Hanoch T, Yung Y, Ridner G, Lando Z, Zharhary D, Seger R. 2000. Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468(1):37-42.

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    • Prof. Rony Seger
    240
    240 - Monoclonal antibody directed to ubiquitinated-H2B Description: Monoclonal antibodies, specific to ubiquitinated-H2B in its ubiquitinated form but not in its unmodified state, or to ubiquitin un-conjugated to H2B. Monoubiquitylated-H2B takes part in almost every molecular process associated with...

    240 - Monoclonal antibody directed to ubiquitinated-H2B

    Description: Monoclonal antibodies, specific to ubiquitinated-H2B in its ubiquitinated form but not in its unmodified state, or to ubiquitin un-conjugated to H2B. Monoubiquitylated-H2B takes part in almost every molecular process associated with chromatin biology Including transcription initiation and elongation ,DNA damage response and repair, DNA replication, nucleosome positioning, RNA processing and export etc. May be used as a detection tool in western blotting, immunoprecipitation and chromatin immunoprecipitation.

    Reference: Shema E, Tirosh I, Aylon Y, Huang J, Ye C, Moskovits N, Raver-Shapira N, Minsky N, Pirngruber J, Tarcic G, Hublarova P, Moyal L, Gana-Weisz M, Shiloh Y, Yarden Y, Johnsen SA, Vojtesek B, Berger SL, Oren M. 2008. The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. Genes Dev. 1;22(19):2664-76.

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    • Prof. Moshe Oren

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